Research Technician II

This Position is Grant Funded

School of Medicine

Established in 1930, Duke University School of Medicine is the youngest of the nation's top medical schools. Ranked sixth among medical schools in the nation, the School takes pride in being an inclusive community of outstanding learners, investigators, clinicians, and staff where interdisciplinary collaboration is embraced and great ideas accelerate translation of fundamental scientific discoveries to improve human health locally and around the globe. Composed of more than 2,600 faculty physicians and researchers, nearly 2,000 students, and more than 6,200 staff, the Duke University School of Medicine along with the Duke University School of Nursing, and Duke University Health System comprise Duke Health, a world-class academic medical center. The Health System encompasses Duke University Hospital, Duke Regional Hospital, Duke Raleigh Hospital, Duke Health Integrated Practice, Duke Primary Care, Duke Home Care and Hospice, Duke Health and Wellness, and multiple affiliations.

RESEARCH TECHNICIAN II

Moody Discovery Laboratory – Duke Human Vaccine Institute

OVERVIEW:

The Moody Discovery Laboratory focuses on understanding the B cell responses during HIV, influenza, and syphilis infection, providing pre-clinical and translational support for downstream human clinical studies that fall within DHVI’s mission of performing research against infectious diseases that impact global health. The lab is a resource for both nonhuman and human cellular phenotyping, flow characterization, staining and analysis at DHVI. Further, the Moody Discovery Laboratory performs a variety of assays to support the DHVI mission; including ELISA and ELLA based assays, RNA/DNA extraction for sequencing, and production of influenza and syphilis proteins. The Research Technician will report directly to the Senior Research Laboratory Manager.

RESPONSIBILITIES:

1. Work with the PI and in harmony with Laboratory Leadership on the Syphilis Program:
   1. Run ELISAs in support of isolating monoclonal antibodies from human and non-human primate samples.
   2. Assist in the production of proteins
      1. Small and large scale

2. Work with the PI and in harmony with Laboratory Leadership to support the Duke Collaborative Influenza Vaccine Innovation Centers (CIVICs) Vaccine Center (DCVC):
   1. Assist in the production of proteins
      1. Small and large scale
   2. Run lab assays
      1. ELISAS
      2. NAI
      3. Protein conjugations
      4. FPLC
      5. Microscopy

3. Adhere to established laboratory SOPs for the performance of duties.

4. Maintain sufficient inventory of material, supplies, and equipment for the performance of duties.

REQUIRED QUALIFICATIONS AT THIS LEVEL:

• Education/Training: Work generally requires a bachelor's degree in botany, biology, zoology, psychology or other directly related scientific field.
• Experience: None required above education/training requirement. OR AN EQUIVALENT COMBINATION OF RELEVANT EDUCATION AND/OR EXPERIENCE.

PREFERENCES:

• Proven organizational skills, communication skills, and proficiency in Microsoft Excel, Word, and PowerPoint.
• Comfortable providing written and oral updates to multiple levels.
• Capable of building strong working relationships with internal and external teams.

Duke is an Affirmative Action/Equal Opportunity Employer committed to providing employment opportunity without regard to an individual's age, color, disability, gender, gender expression, gender identity, genetic information, national origin, race, religion, sex, sexual orientation, or veteran status.

Duke aspires to create a community built on collaboration, innovation, creativity, and belonging. Our collective success depends on the robust exchange of ideas—an exchange that is best when the rich diversity of our perspectives, backgrounds, and experiences flourishes. To achieve this exchange, it is essential that all members of the community feel secure and welcome, that the contributions of all individuals are respected, and that all voices are heard. All members of our community have a responsibility to uphold these values.

Essential Physical Job Functions: Certain jobs at Duke University and Duke University Health System may include essentialjob functions that require specific physical and/or mental abilities. Additional information and provision for requests for reasonable accommodation will be provided by each hiring department.