Research Technician II

Duke Health | Durham, NC, United States

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Posted Date 9/13/2021
Description

This Position is Fully Grant Funded

School of Medicine:

Established in 1930, Duke University School of Medicine is the youngest of the nation’s top medical schools. Ranked tenth among its peers, the School takes pride in being an inclusive community of outstanding learners, investigators, clinicians, and staff where traditional barriers are low, interdisciplinary collaboration is embraced, and great ideas accelerate translation of fundamental scientific discoveries to improve humanhealth locally and around the globe.

Comprised of 2,400 faculty physicians and researchers, the Duke University School of Medicine along with the Duke University School of Nursing and Duke University Health System create Duke Health. Duke Health is a world-class health care network. Founded in 1998 to provide efficient, responsive care, the health system offers a full network of health services and encompasses Duke University Hospital, Duke Regional Hospital, Duke Raleigh Hospital, Duke Primary Care, Private Diagnostic Clinic, Duke Home and Hospice, Duke Health and Wellness, and multiple affiliations.

Occupational Summary:

Implement technical activities of a scientific or research group in relation to the operation of specialized laboratories. Acts as an advisor to subordinates to meet schedules and/or resolve technical problems. Develops assay budgets, schedules, and performance requirements. Frequently interacts with other labs and coordinates the program activities with responsibility for results in terms of costs, methods, and employees. Serve as an advisor to faculty and lab managers to meet schedules and or resolve technical problems. Insure Standard Operating Procedures, GCLP and protocols are maintained and applied.

Work Performed:

  • Perform assays assessing virus-specific responses against HIV
  • Process and isolate mononuclear cells from human and nonhuman primate blood and other tissues and conduct subsequent steps involved in the phenotypic evaluation of cells by flow cytometry, including cellular staining techniques and data acquisition, analysis, and maintenance.
  • Perform ordering responsibilities for the laboratory, including taking inventory, restocking, submitting orders, managing samples, and coordinating with other lab members regarding their specific assay needs.
  • Perform cell culture, virus, and reagent preparation for immunologic and virologic assays
  • Prepare and process specimens for virological procedures, Virus Culture, Real Time PCR, ELISA, Western Blot, and devise experimental techniques for testing and analysis as directed by laboratory manager.
  • Maintain quality checks on safety of laboratories, including biohazards and insure maintenance of the facilities according to Good Clinical Laboratory Practices (GCLP).
  • Assist to maintain and order sufficient inventory of materials currently used laboratory, supplies and equipment for performance of relative assay including plastics ware, media and specified instrument reagents and instrument QC samples.
  • Assist to set up lab equipment used in laboratory related assays. Assist with upkeep such as setup, temperature monitoring, daily verify w/QC, proficiency, operate, perform daily maintenance, troubleshooting, record keeping, etc as instructed
  • Perform proficiency tests on [.e. CAP, IQA, UKNEQAS etc]
  • Setting up & participate in all aspects of the EQA send outs, [labeling tubes, prepare shipping boxes, shipping labels, aliquoting samples, testing samples, data summary for flow samples etc].
  • Perform data summaries, procedural summaries; log keeping, assist in writing up for standard operating procedures, GCLP related policies, methodology, standards, recommendations and QA & QC.
  • Perform other laboratory or data management duties, including laboratory maintenance tasks, as requested by the Principal Investigator or senior lab staff.
  • Collect information and data through observation, experimentation and various other methods, to include operating highly technical laboratory instruments and equipment.
  • Prepare and process specimens for complex immunological and virological procedures, including but not limited to PBMC Processing, Flow Cytometry.

Required Qualifications at this Level

  • Education/Training: Work generally requires a bachelor's degree in botany, biology, zoology, psychology or other directly related scientific field.
  • Experience: None required above education/training requirement. OR AN EQUIVALENT COMBINATION OF RELEVANT EDUCATION AND/OR EXPERIENCE

Duke is an Affirmative Action/Equal Opportunity Employer committed to providing employment opportunity without regard to an individual's age, color, disability, gender, gender expression, gender identity, genetic information, national origin, race, religion, sex, sexual orientation, or veteran status.

Duke aspires to create a community built on collaboration, innovation, creativity, and belonging. Our collective success depends on the robust exchange of ideas—an exchange that is best when the rich diversity of our perspectives, backgrounds, and experiences flourishes. To achieve this exchange, it is essential that all members of the community feel secure and welcome, that the contributions of all individuals are respected, and that all voices are heard. All members of our community have a responsibility to uphold these values.

Essential Physical Job Functions: Certain jobs at Duke University and Duke University Health System may include essentialjob functions that require specific physical and/or mental abilities. Additional information and provision for requests for reasonable accommodation will be provided by each hiring department.

Category
Healthcare & Public Health | Research and Development

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