Research Technician II

Duke Health | Durham, NC, United States

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Posted Date 6/06/2023

This Position is Fully Grant Funded

School of Medicine Established in 1930, Duke University School of Medicine is the youngest of the nation's top medical schools. Ranked sixth among medical schools in the nation, the School takes pride in being an inclusive community of outstanding learners, investigators, clinicians, and staff where interdisciplinary collaboration is embraced and great ideas accelerate translation of fundamental scientific discoveries to improve human health locally and around the globe. Composed of more than 2,500 faculty physicians and researchers, more than 1,300 students, and more than 6,000 staff, the Duke University School of Medicine along with the Duke University School of Nursing, Duke University Health System and the Private Diagnostic Clinic (PDC) comprise Duke Health. a world-class academic medical center. The Health System encompasses Duke University Hospital, Duke Regional Hospital, Duke Raleigh Hospital, Duke Primary Care, Duke Home and Hospice, Duke Health and Wellness, and multiple affiliations.

Occupational Summary

Perform a variety of complextechnical duties involved in conducting high-throughput genomics experiments tosupport research efforts in the Reddy and Crawford Labs a t Duke University. Theposition is funded through an NHGRI awarded Center of Excellence (CEGS) inGenome Science. The focus of the Duke CEGS is to develop new andhigh-throughput technologies to investigate how combinat orial gene regulationcontributes to human health and disease. A multidis ciplinary team of seven Dukeprincipal investigators leads the project. T he research technician hired forthis position will report directly to PI s Dr. Tim Reddy and Dr. Greg Crawfordand will be under the direction and supervision of the graduate students andpostdocs leading the project. A dditionally, the hired individual will be givenample opportunities to fu rther develop independent research skills.

Work Performed

Major tasks to be performed willinclude: prioritization o fsequencing on the identified patient cohortsinvolved with the CEGS pro ject, analysis and troubleshooting sequencing datagenerated from existin g patient and family member samples, discussions withclinical experts an d genetic counselor team members to interpret potential causalvariants, and identify follow-up studies to validate findings. Technologiesinvolve d include preparations for genome and transcriptome sequencing, andlong- read sequencing; preparation of PBMCs, and CRISPR/Cas9 validation studie s.

Perform routine laboratory tasksrelatedto the above goals including preparation of reagent stocks, cell growthmedia, and buf fers; ordering and stocking sufficient supplies and equipment forperform ing experiments; cleaning and maintain laboratory equipment.

Review results and qualitycontrol from completed experiments; record results in lab notebooks;communicate results in weekly update meetings w ith project leads. Option tocontribute in weekly journal clubs and group meetings.

Perform other tasks and specialindepende nt projects as assigned.

Minimum Qualifications


Work generally requires a bachelor's degree in botany, biology, zoology, psychology or other directly related scientific field.


None required above education/training requirement. OR AN EQUIVALENT COMBINATION OF RELEVANT EDUCATION AND/OR EXPERIENCE

Duke is an Affirmative Action/Equal Opportunity Employer committed to providing employment opportunity without regard to an individual's age, color, disability, gender, gender expression, gender identity, genetic information, national origin, race, religion, sex, sexual orientation, or veteran status.

Duke aspires to create a community built on collaboration, innovation, creativity, and belonging. Our collective success depends on the robust exchange of ideas—an exchange that is best when the rich diversity of our perspectives, backgrounds, and experiences flourishes. To achieve this exchange, it is essential that all members of the community feel secure and welcome, that the contributions of all individuals are respected, and that all voices are heard. All members of our community have a responsibility to uphold these values.

Essential Physical Job Functions: Certain jobs at Duke University and Duke University Health System may include essentialjob functions that require specific physical and/or mental abilities. Additional information and provision for requests for reasonable accommodation will be provided by each hiring department.

Healthcare & Public Health | Research and Development

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